gene targeting Search Results


target gene  (Omics Data Automation)
86
Omics Data Automation target gene
Target Gene, supplied by Omics Data Automation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pm41166154-131-64-68?v=Omics+Data+Automation
Average 86 stars, based on 1 article reviews
target gene - by Bioz Stars, 2026-06
86/100 stars
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sars cov 2 target genes  (Revvity)
90
Revvity sars cov 2 target genes
Sars Cov 2 Target Genes, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pmc08685303-101-12-1?v=Revvity
Average 90 stars, based on 1 article reviews
sars cov 2 target genes - by Bioz Stars, 2026-06
90/100 stars
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reporter gene mice  (Jackson Laboratory)
86
Jackson Laboratory reporter gene mice
Reporter Gene Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/10__1523_slash_jneurosci__2130___22__2023-43-1-7?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
reporter gene mice - by Bioz Stars, 2026-06
86/100 stars
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cdc20 target gene  (SAS institute)
90
SAS institute cdc20 target gene
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
Cdc20 Target Gene, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pmc08798816-124-10-12?v=SAS+institute
Average 90 stars, based on 1 article reviews
cdc20 target gene - by Bioz Stars, 2026-06
90/100 stars
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shrna of lad1  (Shanghai GenePharma)
90
Shanghai GenePharma shrna of lad1
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
Shrna Of Lad1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pm37718450-68-3-7?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
shrna of lad1 - by Bioz Stars, 2026-06
90/100 stars
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primers targeting dna polymerase i (pola) gene  (TIB MOLBIOL)
90
TIB MOLBIOL primers targeting dna polymerase i (pola) gene
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
Primers Targeting Dna Polymerase I (Pola) Gene, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pm34780379-47-13-28?v=TIB+MOLBIOL
Average 90 stars, based on 1 article reviews
primers targeting dna polymerase i (pola) gene - by Bioz Stars, 2026-06
90/100 stars
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gene-targeted embryonic stem cells  (Regeneron inc)
90
Regeneron inc gene-targeted embryonic stem cells
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
Gene Targeted Embryonic Stem Cells, supplied by Regeneron inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pmc03589669-364-21-5?v=Regeneron+inc
Average 90 stars, based on 1 article reviews
gene-targeted embryonic stem cells - by Bioz Stars, 2026-06
90/100 stars
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sirna duplexes targeting human p55ik gene (si-p55ik)  (Ribobio co)
90
Ribobio co sirna duplexes targeting human p55ik gene (si-p55ik)
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
Sirna Duplexes Targeting Human P55ik Gene (Si P55ik), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pm24632606-144-13-33?v=Ribobio+co
Average 90 stars, based on 1 article reviews
sirna duplexes targeting human p55ik gene (si-p55ik) - by Bioz Stars, 2026-06
90/100 stars
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neomycin phosphotransferase (neor) gene expression cassette  (inGenious Targeting Laboratory)
90
inGenious Targeting Laboratory neomycin phosphotransferase (neor) gene expression cassette
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
Neomycin Phosphotransferase (Neor) Gene Expression Cassette, supplied by inGenious Targeting Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pm24635668-50-1-33?v=inGenious+Targeting+Laboratory
Average 90 stars, based on 1 article reviews
neomycin phosphotransferase (neor) gene expression cassette - by Bioz Stars, 2026-06
90/100 stars
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rt-qpcr kits targeting the e-gene  (TIB MOLBIOL)
90
TIB MOLBIOL rt-qpcr kits targeting the e-gene
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
Rt Qpcr Kits Targeting The E Gene, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pmc07307078-88-31-34?v=TIB+MOLBIOL
Average 90 stars, based on 1 article reviews
rt-qpcr kits targeting the e-gene - by Bioz Stars, 2026-06
90/100 stars
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target gene - fragmentation oligo pool  (PrimerDesign Inc)
90
PrimerDesign Inc target gene - fragmentation oligo pool
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
Target Gene Fragmentation Oligo Pool, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pmc06954442__gkz1110_supplemental_file-0-0-17?v=PrimerDesign+Inc
Average 90 stars, based on 1 article reviews
target gene - fragmentation oligo pool - by Bioz Stars, 2026-06
90/100 stars
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16s dna profile targeting the v4 region of the ssu rrna gene  (Broad Institute Inc)
90
Broad Institute Inc 16s dna profile targeting the v4 region of the ssu rrna gene
Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , <t>CDC20</t> , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .
16s Dna Profile Targeting The V4 Region Of The Ssu Rrna Gene, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+targeting/pmc06018966-119-14-7?v=Broad+Institute+Inc
Average 90 stars, based on 1 article reviews
16s dna profile targeting the v4 region of the ssu rrna gene - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , CDC20 , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .

Journal: Translational Cancer Research

Article Title: Analysis of novel enzalutamide-resistant cells: upregulation of testis-specific Y-encoded protein gene promotes the expression of androgen receptor splicing variant 7

doi: 10.21037/tcr-20-1463

Figure Lengend Snippet: Characterization of AR status and expression of AR target genes in SAS MDV No. 3–14 cells. (A) LNCaP and SAS MDV No. 3–14 cells were cultured under androgen-deprived conditions for 1 day. Total RNA was extracted from cells and cDNA was synthesized. The mRNA expression of AR , AR-v7 , and GAPDH was analyzed by qRT-PCR analysis. The expression of AR and AR-v7 was normalized to the corresponding expression of GAPDH , and the relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, and a GAPDH antibody were used for protein detection. (C) The mRNA expression of KLK3 , FKBP5 , TMPRSS2 , CDK1 , CDC20 , and UBE2C in LNCaP or SAS MDV No. 3–14 cells was analyzed by qRT-PCR analysis. The level of each target gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). ***, P<0.001. AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; UBE2C , ubiquitin conjugating enzyme E2 C .

Article Snippet: In addition, the expression of UBE2C , CDK1 , and CDC20 in SAS MDV No. 3–14 cells was higher than that in LNCaP cells ( ).

Techniques: Expressing, Cell Culture, Synthesized, Quantitative RT-PCR, Western Blot, Ubiquitin Proteomics

The function of TSPY in the proliferation, expression and transcriptional activity of AR in SAS MDV No. 3–14 cells. (A) The mRNA expression of TSPY in indicated cell lines was analyzed by qRT-PCR analysis. The expression of the TSPY gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole- cell lysates of indicated cell lines were harvested and subjected to immunoblotting analysis. The TSPY and GAPDH antibodies were used as primary antibodies, respectively. (C) SAS MDV No. 3–14 cells were transfected with negative control (NGT) or TSPY siRNA under androgen-deprived conditions. Three days after transfection with siRNA, cell proliferation was evaluated using the CellTiter-Glo assay. Data represent mean ± SD (N=3). (D,E) Three days after transfection with siRNA, whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, a TSPY antibody, and a GAPDH antibody were used as primary antibodies. The expression of TSPY , AR , AR-v7 , KLK3 , FKBP5 , TMPRSS2 , CDK1 , CDC20 , and UBE2C genes was analyzed by qRT-PCR analysis. The expression of each gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in the NGT-treatment group was set as 1. Data represent mean ± SD (N=3). *, P<0.05; **, P<0.01; ***, P<0.001. n.s., not significant; AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; NGT, negative control; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; TSPY , testis-specific Y-encoded protein; UBE2C , ubiquitin conjugating enzyme E2 C .

Journal: Translational Cancer Research

Article Title: Analysis of novel enzalutamide-resistant cells: upregulation of testis-specific Y-encoded protein gene promotes the expression of androgen receptor splicing variant 7

doi: 10.21037/tcr-20-1463

Figure Lengend Snippet: The function of TSPY in the proliferation, expression and transcriptional activity of AR in SAS MDV No. 3–14 cells. (A) The mRNA expression of TSPY in indicated cell lines was analyzed by qRT-PCR analysis. The expression of the TSPY gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in LNCaP cells was set as 1. Data represent mean ± SD (N=3). (B) Whole- cell lysates of indicated cell lines were harvested and subjected to immunoblotting analysis. The TSPY and GAPDH antibodies were used as primary antibodies, respectively. (C) SAS MDV No. 3–14 cells were transfected with negative control (NGT) or TSPY siRNA under androgen-deprived conditions. Three days after transfection with siRNA, cell proliferation was evaluated using the CellTiter-Glo assay. Data represent mean ± SD (N=3). (D,E) Three days after transfection with siRNA, whole-cell lysates were harvested and subjected to immunoblotting analysis. An AR antibody recognizing the AR n-terminal domain (AR D6F11), an AR-v7 specific antibody, a TSPY antibody, and a GAPDH antibody were used as primary antibodies. The expression of TSPY , AR , AR-v7 , KLK3 , FKBP5 , TMPRSS2 , CDK1 , CDC20 , and UBE2C genes was analyzed by qRT-PCR analysis. The expression of each gene was normalized to the corresponding expression of GAPDH, and relative mRNA expression in the NGT-treatment group was set as 1. Data represent mean ± SD (N=3). *, P<0.05; **, P<0.01; ***, P<0.001. n.s., not significant; AR, androgen receptor; GAPDH , glyceraldehyde 3-phosphate dehydrogenase; NGT, negative control; KLK3 , kallikrein-3; FKBP5 , FKBP prolyl isomerase 5; TMPRSS2 , transmembrane serine protease 2; CDK1 , cyclin-dependent kinase 1; CDC20 , cell division cycle 20; TSPY , testis-specific Y-encoded protein; UBE2C , ubiquitin conjugating enzyme E2 C .

Article Snippet: In addition, the expression of UBE2C , CDK1 , and CDC20 in SAS MDV No. 3–14 cells was higher than that in LNCaP cells ( ).

Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Western Blot, Transfection, Negative Control, Glo Assay, Ubiquitin Proteomics